A Virulence-Associated, 6.4-kb, Double-Stranded RNA from Rhizoctonia solani Is Phylogenetically Related to Plant Bromoviruses and Electron Transport Enzymes

A Virulence-Associated, 6.4-kb, Double-Stranded RNA from Rhizoctonia solani Is Phylogenetically Related to Plant Bromoviruses and Electron Transport Enzymes

Blog Article

We have recently shown that acquisition of a 6.4-kb, double-stranded (ds) RNA (M1) by hyphal anastomosis is associated with enhanced vigor and virulence, whereas its removal by hyphal tipping correlates with diminished virulence in the life extension blueberry extract plant-pathogenic basidiomycete Rhizoctonia solani.The M1 dsRNA is not encapsidated by a typical nucleocapsid, has a circular and/or concatemeric form, and is associated with the mitochondrial and cytosolic fractions.M1 possesses six open reading frames (ORFs) the longest of which (ORF 2) is located on the (+) strand, and encodes a putative polypeptide consisting of 1,747 amino acids or 199.

4 turbosound ts-18sw700/8a kDa.This polypeptide has a significant amino acid sequence similarity, including six conserved helicase domains and an ATP/GTP binding motif, with the 1A protein of broad bean mottle virus (BBMV) and other bromoviruses.ORF 5, which is located on the (-) strand of M1 and is complementary to a region of ORF 2, codes for a putative polypeptide that has a significant amino acid sequence similarity with the cytochrome c oxidase assembly factor.This complementarity provides direct evidence suggesting that the long-standing hypothesis of viruses evolving from cellular genes may be valid.